Human Foreskins Sold for Human Embryonic Stem Cell Research,
Cloning, and the Creation of Chimeras (Animal/Human Hybrids)

Aug. 15, 2003
"Scientists in China have created the world's first embryonic chimeras,
hybrid embryos that contain human and rabbit DNA, according to the
Journal of Cell Research.  The Greek mythological creature called the
chimera was a fire-breathing she-monster, with the head of a lion, the
body of a goat and a serpent for a tail. A chimera has come to mean any
hybrid of two or more creatures and is a term used in genetics.  In the
Chinese experiment, human nuclei was extracted from foreskin tissue and
facial tissue from males and females ages 5-60. Samples were obtained
from tissue discarded after surgery."


Reference:
http://www.newsmax.com/archives/articles/2003/8/14/153903.shtml


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"THE age of embryonic stem cells edges closer. Researchers have found a
way to create many personalised ESCs from a single embryo . . .  Now
Kevin Eggan's team at Harvard University has dispensed with the egg and
replaced it with ESCs from a spare IVF embryo that would have been
discarded. To these they fused a man's foreskin cells, creating what
appear to be new personalised ESCs (Science, vol 309, p 1369)."


Reference:
http://www.newscientist.com/article/mg18725143.500.html

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"Endogenous expression of a high-affinity pseudoknot RNA aptamer
suppresses replication of HIV-1


Laurent Chaloin, Maik Jörg Lehmann1, Georg Sczakiel2 and Tobias Restle*
Max-Planck-Institut für molekulare Physiologie, Abteilung Physikalische
Biochemie, Otto Hahn Straße 11, 44227 Dortmund, Germany, 1 Deutsches
Krebsforschungszentrum, Forschungsschwerpunkt Angewandte Tumorvirologie,
Im Neuenheimer Feld 242, 69120 Heidelberg, Germany and 2 Institut für
Molekulare Medizin, Medizinische Universität zu Lübeck, Ratzeburger Allee
160, 23538 Lübeck, Germany"


Cell lines and cell tissue culture
HS 68 (human newborn foreskin fibroblast) and 293T (human embryonic
kidney cells) cell lines were cultured in Dulbecco's Modified Eagle's
Medium (DMEM; Life Technologies, Karlsruhe, Germany), supplemented with
10% fetal calf serum, 2 mM L-glutamine, 100 IU/ml penicillin and 100
µg/ml streptomycin at 37°C in a humidified atmosphere containing 5% CO2 .
 .


Reference:  http://nar.oxfordjournals.org/cgi/content/full/30/18/4001

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Neonatal Foreskin Epidermal Keratinocytes
Price: $250
Product Summary
Description  Epidermal keratinocytes- pooled, cultures established on
Collagen IV
Derivation  Neonatal foreskins
Coriell Institute for Medical Research
403 Haddon Avenue
Camden, NJ 08103


Reference:  http://ccr.coriell.org/ccr/products/nfek.html

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Stem Cell Research
Human foreskin feeders can nourish human embryonic stem cells in culture
Source: Gene Therapy Weekly (2003-07-24)


Reference:
http://www.newsrx.com/newsletters/Gene-Therapy-Weekly/2003-07-24/072420033334GW.html

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American Type Culture Collection (ATCC)
P.O. Box 1549
Manassas, VA 20108


Each lot of feeder cells from the ATCC Stem Cell Center has been
rigorously tested to support the growth of embryonic stem cells.
Order 6 or more vials and get special pricing!
Description Strain ATCC® No. Price per vial , 6 or more


HFF-1 foreskin fibroblasts, newborn SCRC-1041ä $100 $80
HFF-1 foreskin fibroblasts, irradiated SCRC-1041.1ä $50 $45
HFF-1 foreskin fibroblasts, mitomycin C treated SCRC-1041.2ä $50 $45
HFF-2 foreskin fibroblasts, newborn SCRC-1042ä $100 $80
HFF-2 foreskin fibroblasts, mitomycin C treated SCRC-1042.2ä $50 $45


Reference:  http://www.stemcells.atcc.org

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"A culture system using human foreskin fibroblasts as feeder cells allows
production of human embryonic stem cells


Authors: Outi Hovatta1; Milla Mikkola1; Karin Gertow2; Anne-Marie
Strömberg1; José Inzunza1; Julius Hreinsson1; Björn Rozell2; Elisabeth
Blennow3; Michael Andäng1; Lars Ährlund-Richter2Source: Human
Reproduction, Volume 18, Number 7, July 2003, pp. 1404-1409(6)Publisher:
OxfordUniversity Press


Abstract:  . . . METHODS: We used post-natal, commercially available
human foreskin fibroblasts as feeder cells. Inner cell masses (ICM) were
isolated from five supernumerary blastocysts, obtained as donations from
couples undergoing IVF treatment."


Reference:
http://www.ingentaconnect.com/content/oup/humrep/2003/00000018/00000007/art01404

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Product Name:  Fibroblasts/Epithelial cells antibody [D7-FIB]
Product Type:  Primary antibodies
Description:  Mouse monoclonal [D7-FIB] to Fibroblasts/Epithelial cells
Immunogen:  Human foreskin fibroblasts


Reference:
http://abcam.com/index.html?pageconfig=datasheet&intAbID=11208